Method | Recognition Criteria |
---|---|
bright-field, Nomarski contrast microscopy | nucleus appears as a restricted compartment with a granular inside |
phase contrast microscopy | nucleus appears as a restricted compartment with coarse inner material surrounded by a refracting sheath |
bright-field microscopy of fixed tissue stained using carmine or hematoxylin | nucleus appears as a restricted compartment with clustered or completely red (carmine) or black (hematoxylin) pigmentation |
transmission electron microscopy, stained with heavy metals | nucleus appears as a membrane-bound compartment scattered irregularly with electron-dense clusters of varying size to complete electron-dense staining |